Goat Thrombomodulin ELISA Kit | TM elisa kit
Goat Thrombomodulin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This TM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TM. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY TM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TM antibody and an TM-HRP conjugate. The assay sample and buffer are incubated together with TM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TM concentration since TM from samples and TM-HRP conjugate compete for the anti-TM antibody binding site. Since the number of sites is limited, as more sites are occupied by TM from the sample, fewer sites are left to bind TM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
Uniprot Description
thrombomodulin: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THPH12). A hemostatic disorder characterized by a tendency to thrombosis. Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 20p11.2
Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; calcium ion binding
Biological Process: response to cAMP; negative regulation of fibrinolysis; negative regulation of blood coagulation; response to lipopolysaccharide; female pregnancy; signal transduction; blood coagulation; leukocyte migration; response to X-ray
Disease: Thrombophilia Due To Thrombomodulin Defect; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 6
