Guinea Pig Toll-Like Receptor 4 ELISA Kit | TLR4 elisa kit

Guinea pig TLR4 (Toll-Like Receptor 4) ELISA Kit

Cat. Num. AAA2533637

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Reactivity: Guinea Pig
• Synonyms: Toll-Like Receptor 4; Guinea pig TLR4 (Toll-Like Receptor 4) ELISA Kit; TLR4 elisa kit

• Reactivity: Guinea Pig
• Specificity: This kit recognizes natural and recombinantGuinea pigTLR4. No significant cross-reactivity or interference between Guinea pigTLR4 and analogues was observed.
• Sequence Length: 839

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR4 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of Guinea pigTLR4concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TLR4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TLR4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TLR4.You can calculate the concentration of TLR4in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19924149
• NCBI GeneID: 7099
• NCBI Accession #: NP_612564.1
• NCBI GenBank Nucleotide #: NM_138554.4
• Molecular Weight: 73,301 Da
• NCBI Official Full Name: toll-like receptor 4 isoform A
• NCBI Official Synonym Full Names: toll like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR4
• UniProt Entry Name: TLR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cell surface; cytoplasm; endosome membrane; external side of plasma membrane; integral to plasma membrane; intrinsic to plasma membrane; lipopolysaccharide receptor complex; perinuclear region of cytoplasm; plasma membrane

Molecular Function: lipopolysaccharide binding; lipopolysaccharide receptor activity; protein binding; receptor activity; transmembrane receptor activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; apoptosis; astrocyte development; B cell proliferation during immune response; defense response to bacterium; defense response to Gram-negative bacterium; detection of fungus; detection of lipopolysaccharide; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; immune response; innate immune response; interferon-gamma production; interleukin-1 beta secretion; lipopolysaccharide-mediated signaling pathway; macrophage activation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; negative regulation of interleukin-23 production; negative regulation of interleukin-6 production; negative regulation of osteoclast differentiation; negative regulation of tumor necrosis factor production; positive regulation of B cell proliferation; positive regulation of chemokine production; positive regulation of inflammatory response; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of interferon-gamma production; positive regulation of interleukin-1 production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of MHC class II biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; production of nitric oxide during acute inflammatory response; programmed cell death; regulation of cytokine secretion; regulation of dendritic cell cytokine production; response to lipopolysaccharide; T-helper 1 type immune response; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway

Disease: Macular Degeneration, Age-related, 10