Toll Like Receptor 3 (TLR3) Recombinant Protein | TLR3 recombinant protein

Recombinant Toll Like Receptor 3 (TLR3)

Cat. Num. AAA2018768

• Gene Names: Tlr3; AI957183
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Toll Like Receptor 3 (TLR3); Recombinant Toll Like Receptor 3 (TLR3); TLR3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-EGWR ISFYWNVSVH RILGFKEIDT QAEQFEYTAY IIHAHKDRDW VWEHFSPMEE QDQSLKFCLE ERDFEAGVLG LEAIVNSIKR SRKIIFVITH HLLKDPLCRR FKVHHAVQQA IEQNLDSIIL IFLQNIPDYKLNHALCLRRG MFKSHCILNW PVQKERINAF HHKLQVALGS RNSAH
• Sequence Length: 905

• Applicable Applications for TLR3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu727~His905 (Accession # Q99MB1) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 31543872
• NCBI GeneID: 142980
• NCBI Accession #: NP_569054.2
• NCBI GenBank Nucleotide #: NM_126166.4
• UniProt Accession #: Q99MB1; Q91ZM4
• Molecular Weight: 53.4kDa
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: Tlr3
• NCBI Official Synonym Symbols: AI957183
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr3
• UniProt Entry Name: TLR3_MOUSE

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: cell surface; membrane; lysosomal membrane; endoplasmic reticulum; cytoplasm; integral to membrane; intracellular; endosome

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; RNA binding; double-stranded RNA binding; receptor activity

Biological Process: positive regulation of interleukin-12 production; positive regulation of JNK cascade; microglial cell activation; defense response; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; inflammatory response; defense response to virus; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; MyD88-independent toll-like receptor signaling pathway; response to virus; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response