Talin 2 Recombinant Protein | TLN2 recombinant protein

Recombinant Talin 2 (TLN2)

Cat. Num. AAA2033286

• Gene Names: TLN2; ILWEQ
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Talin 2; Recombinant Talin 2 (TLN2); ILWEQ; TLN2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: F GTECTLGYLDLLEHVLVILQ KPTPEFKQQL AAFSKRVAGA VTELIQAAEA MKGTEWVDPEDPTVIAETEL LGAAASIEAA AKKLEQLKPR AKPKQADETL DFEEQILEAAKSIAAATSAL VKSASAAQRE LVAQGKVGSI PANAADDGQW SQGLISAARMVAAATSSLCE AANASVQGHA SEEKLISSAK QVAASTAQLL VACKVKADQDSEAMRRLQAA GNAVKRASDN LVRAAQKAAF GKADDDDVVV KT
• Sequence Length: 2542

• Applicable Applications for TLN2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 30.1kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Phe2240~Thr2492
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21666571
• NCBI GeneID: 83660
• Molecular Weight: 271,613 Da
• NCBI Official Full Name: talin 2
• NCBI Official Synonym Full Names: talin 2
• NCBI Official Symbol: TLN2
• NCBI Official Synonym Symbols: ILWEQ
• NCBI Protein Information: talin-2
• UniProt Protein Name: Talin-2
• Protein Family: Talin
• UniProt Gene Name: TLN2
• UniProt Synonym Gene Names: KIAA0320
• UniProt Entry Name: TLN2_HUMAN

NCBI Description

This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

talin 2: As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: actin cytoskeleton; cytoplasm; fascia adherens; focal adhesion; intercellular junction; plasma membrane; ruffle; synapse

Molecular Function: actin binding; actin filament binding; protein binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: cell adhesion; cytoskeletal anchoring; intercellular junction assembly