TLE3 recombinant protein

TLE3 Protein, Human, Recombinant (aa 484-772, GST Tag)

Cat. Num. AAA8121272

• Gene Names: TLE3; ESG; ESG3; GRG3; HsT18976
• Purity: >78% as determined by SDS-PAGE
• Synonyms: TLE3; TLE3 Protein; Human; Recombinant (aa 484-772; GST Tag); Human TLE3 Protein (aa 484-772; ESG Protein; ESG3 Protein; GRG3 Protein; HsT18976 Protein; transducin-like enhancer of split 3; TLE3 recombinant protein

• Host: E Coli
• Purity/Purification: >78% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.15M NaCl, 5mM GSH, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser484-Tyr772

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the N-terminal fragment of human TLE3 (Q04726-1)(Ser 484-Tyr 772) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TLE3 recombinant protein

Background: The association between high TLE3 expression and a favorable response to taxane-containing chemotherapy regimens was validated in patients with non-serous ovarian cancer. That TLE3 expression may serve as a marker of chemosensitivity in taxane-treated patients with non-serous histologies. Transducin-like enhancer of Split3 (TLE3) serves as a transcriptional corepressor during cell differentiation and shows multiple roles in different kinds of cancers. TLE3 repressed CRC proliferation partly through inhibition of MAPK and AKT signaling pathways, suggesting the possibility of TLE3 as a biomarker for CRC prognosis.

References

Villanueva CJ, et al. (2011) TLE3 is a dual-function transcriptional coregulator of adipogenesis. Cell Metab. 13(4): 413-27.Carvalho LR, et al. (2010) Corepressors TLE1 and TLE3 interact with HESX1 and PROP1. Mol Endocrinol. 24(4): 754-65.Nakaya HI, et al. (2007) Splice variants of TLE family genes and up-regulation of a TLE3 isoform in prostate tumors. Biochem Biophys Res Commun. 364(4): 918-23.Brinkmeier ML, et al. (2003) TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17: 2152-2161.Dasen JS, et al. (2001) Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis. Genes Dev. 15: 3193-3207.Douglas KR, et al. (2001) Identification of members of the Wnt signaling pathway in the embryonic pituitary gland. Mamm Genome. 12: 843-851.Chen G, et al. (2000) Groucho/TLE family proteins and transcriptional repression. Gene. 249: 1-16.

NCBI and Uniprot Product Information

• NCBI GI #: 20532417
• NCBI GeneID: 7090
• UniProt Accession #: Q04726; Q6PI57; Q8IVV6; Q8WVR2; Q9HCM5; B4DPT0; E9PD64; F8W964
• Molecular Weight: 772
• NCBI Official Full Name: Transducin-like enhancer protein 3
• NCBI Official Synonym Full Names: transducin-like enhancer of split 3
• NCBI Official Symbol: TLE3
• NCBI Official Synonym Symbols: ESG; ESG3; GRG3; HsT18976
• NCBI Protein Information: transducin-like enhancer protein 3; enhancer of split groucho 3; enhancer of split groucho-like protein 3; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1)
• UniProt Protein Name: Transducin-like enhancer protein 3
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE3
• UniProt Synonym Gene Names: KIAA1547; ESG3
• UniProt Entry Name: TLE3_HUMAN

NCBI Description

This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

TLE3: transcriptional corepressor that binds to a number of transcription factors. Belongs to the WD-repeat Groucho/TLE family. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Four alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: Notch signaling pathway; organ morphogenesis; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; signal transduction