herpesvirus 1 Thymidine kinase (TK) Recombinant Protein | TK recombinant protein

Recombinant Human herpesvirus 1 Thymidine kinase (TK)

Cat. Num. AAA1237171

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: herpesvirus 1 Thymidine kinase (TK); Recombinant Human herpesvirus 1 Thymidine kinase (TK); TK recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-376aa; Full Length
• Sequence: MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for TK recombinant protein

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.

References

The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.McKnight S.L.Nucleic Acids Res. 8:5949-5964(1980) The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.J. Gen. Virol. 69:1531-1574(1988) The three-dimensional structure of thymidine kinase from herpes simplex virus type 1.Wild K., Bohner T., Aubry A., Folkers G., Schulz G.E.FEBS Lett. 368:289-292(1995) Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir.Brown D.G., Visse R., Sandhu G., Davies A., Rizkallah P.J., Melitz C., Summers W.C., Sanderson M.R.Nat. Struct. Biol. 2:876-881(1995) The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue.Wild K., Bohner T., Folkers G., Schulz G.E.Protein Sci. 6:2097-2106(1997) Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands.Champness J.N., Bennett M.S., Wien F., Visse R., Summers W.C., Herdewijn P., de Clerq E., Ostrowski T., Jarvest R.L., Sanderson M.R.3.0.CO;2-8>Proteins 32:350-361(1998) Structure to 1.9-A resolution of a complex with herpes simplex virus type-1 thymidine kinase of a novel, non-substrate inhibitor X-ray crystallographic comparison with binding of aciclovir.Bennett M.S., Wien F., Champness J.N., Batuwangala T., Rutherford T., Summers W.C., Sun H., Wright G., Sanderson M.R.FEBS Lett. 443:121-125(1999) Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed by X-ray crystallography.Vogt J., Perozzo R., Pautsch A., Prota A., Schelling P., Pilger B., Folkers G., Scapozza L., Schulz G.E.3.0.CO;2-8>Proteins 41:545-553(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 820945252
• NCBI GeneID: 24271467
• NCBI Accession #: YP_009137097.1
• NCBI GenBank Nucleotide #: NC_001806.2
• UniProt Accession #: P03176
• Molecular Weight: 45 kDa
• NCBI Official Full Name: thymidine kinase
• NCBI Official Symbol: UL23
• NCBI Protein Information: involved in nucleotide metabolism; thymidine kinase
• UniProt Protein Name: Thymidine kinase
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK
• UniProt Entry Name: KITH_HHV11

Uniprot Description

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.