Tissue, Membrane Protein Adult Normal, Skin Tissue Lysate
Tissue, Membrane Protein, Human Adult Normal, Skin
Applications
Western Blot
Purity
Molecular Biology Grade
Synonyms
Tissue; Membrane Protein Adult Normal; Skin; Membrane Protein; Human Adult Normal; Skin tissue lysate
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
~2mg/ml (varies by lot)
Applicable Applications for Tissue, Membrane Protein Adult Normal, Skin tissue lysate
Western Blot: 10-20ug/lane
Optimal diutions to be determine by researcher.
Optimal diutions to be determine by researcher.
Application Notes
Electrophoresis, and Immunoprecipitation, , Enzymatic Activity, , Protein-DNA Interaction, , Protein-Protein Interaction, , Tissue-Specific Expression Identification
Product Note
All tissue protein samples are supplied with verified donor age and sex.
Donor Information
1 donor, 45 year-old, normal human female.
Preparation
Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and the stored at -70 degree C.
Quality Control
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the orignal vial after thawing and prior to removing the cap.
Related Product Information for Tissue, Membrane Protein Adult Normal, Skin tissue lysate
Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Similar Products
Product Notes
The Tissue, Membrane Protein Adult Normal, Skin (Catalog #AAA639823) is a Tissue Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tissue, Membrane Protein Adult Normal, Skin can be used in a range of immunoassay formats including, but not limited to, Western Blot: 10-20ug/lane Optimal diutions to be determine by researcher. Electrophoresis, and Immunoprecipitation,, Enzymatic Activity,, Protein-DNA Interaction,, Protein-Protein Interaction,, Tissue-Specific Expression Identification. Researchers should empirically determine the suitability of the Tissue, Membrane Protein Adult Normal, Skin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tissue, Membrane Protein Adult Normal, Skin, Tissue Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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