Thrombospondin 4 (THBS4) Recombinant Protein | THBS4 recombinant protein

Recombinant Thrombospondin 4 (THBS4)

Cat. Num. AAA2011790

• Gene Names: Thbs4; TSP4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thrombospondin 4 (THBS4); Recombinant Thrombospondin 4 (THBS4); THBS4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-GDECDDDDD NDGIPDLVPP GPDNCRLVPN PAQEDSNNDG VGDICEADFD QDQVIDHIDV CPENAEITLT DFRAYQTVVL DPEGDAQIDP NWVVLNQGME IVQTMNSDPG LAVGYTAFNG VDFEGTFHVN TQTDDDYAGF IFGYQDSSSF YVVMWKQTEQ TYWQATPFRA VAEPGIQLKA VKSKTGPGEH LRNSLWHTGD TSDQVRLLWK DSRNVGWKDK VSYRWFLQHR PQVGYIRVRF YEGSELVADS GVTIDTTMRG GRL
• Sequence Length: 963

• Applicable Applications for THBS4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly662~Leu923 (Accession # Q9Z1T2) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6755781
• NCBI GeneID: 21828
• NCBI Accession #: NP_035712.1
• NCBI GenBank Nucleotide #: NM_011582.3
• UniProt Accession #: Q9Z1T2; Q9QYS3; Q9WUE0
• Molecular Weight: 56.4kDa
• NCBI Official Full Name: thrombospondin-4
• NCBI Official Synonym Full Names: thrombospondin 4
• NCBI Official Symbol: Thbs4
• NCBI Official Synonym Symbols: TSP4
• NCBI Protein Information: thrombospondin-4
• UniProt Protein Name: Thrombospondin-4
• Protein Family: Thrombospondin
• UniProt Gene Name: Thbs4
• UniProt Synonym Gene Names: Tsp4
• UniProt Entry Name: TSP4_MOUSE

Uniprot Description

THBS4: Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; sarcoplasmic reticulum; endoplasmic reticulum; extracellular region; basement membrane; neuromuscular junction

Molecular Function: heparin binding; laminin-1 binding; integrin binding; collagen binding; protein binding; growth factor activity; fibronectin binding; calcium ion binding

Biological Process: nervous system development; behavioral response to pain; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell division; myoblast migration; tissue remodeling; positive regulation of endothelial cell proliferation; cell adhesion; response to unfolded protein; protein homooligomerization; neurite morphogenesis; regulation of tissue remodeling