Human thrombospondin 1 ELISA Kit | THBS1 elisa kit

Human thrombospondin 1, TSP-1 ELISA Kit

Cat. Num. AAA701627

• Gene Names: THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
• Reactivity: Human
• Synonyms: thrombospondin 1; Human thrombospondin 1; TSP-1 ELISA Kit; THBS; THBS-1; TSP; TSP-1; TSP1; thrombospondin-1p180; THBS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TSP-1. No significant cross-reactivity or interference between human TSP-1 and analogues was observed.
• Sequence Length: 1170

• Samples: Serum, plasma, breast milk, saliva, tissue homogenates, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 2.7 ng/ml-2000 ng/ml
• Sensitivity: < 2.7 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for THBS1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 40317626
• NCBI GeneID: 7057
• NCBI Accession #: NP_003237.2
• NCBI GenBank Nucleotide #: NM_003246.2
• UniProt Accession #: P07996; Q15667; Q59E99; A8K6H4; B4E3J7; B9EGH6
• Molecular Weight: 129,383 Da
• NCBI Official Full Name: thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: THBS1
• NCBI Official Synonym Symbols: TSP; THBS; TSP1; TSP-1; THBS-1
• NCBI Protein Information: thrombospondin-1; thrombospondin-1p180
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS1
• UniProt Synonym Gene Names: TSP; TSP1
• UniProt Entry Name: TSP1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors 2 PublicationsManual assertion based on experiment in:Ref.13