SDS-Page
THAP1 recombinant protein
THAP1, 1-213aa, Human, His tag, E Coli (Denatured)
Gene Names
THAP1; DYT6
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
THAP1; 1-213aa; Human; His tag; E Coli (Denatured); THAP domain-containing protein 1; DYT6; THAP1 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2M urea, 10% glycerol, 1mM DTT, 0.2M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFK PTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAI GLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV EKLRKKLKTA QQRCRRQERQ LEKLKEVVHF QKEKDDVSER GYVILPNDYF EIVEVPA
Sequence Length
213
Applicable Applications for THAP1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for THAP1 recombinant protein
THAP domain-containing protein 1, also known as THAP1, is a 213 amino acid protein that localizes to the nucleoplasm and contains one THAPtype zinc finger, a conserved DNA-binding domain. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Recombinant human THAP1protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for THAP1 recombinant protein
References
Roussigne M., et al. (2003) Oncogene 22:2432-2442.; Clouaire T., et al. (2005) Proc Natl Acad Sci. 102:6907-6912.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27.5 kDa (237aa)
NCBI Official Full Name
THAP domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
THAP domain containing 1
NCBI Official Symbol
THAP1
NCBI Official Synonym Symbols
DYT6
NCBI Protein Information
THAP domain-containing protein 1
UniProt Protein Name
THAP domain-containing protein 1
Protein Family
UniProt Gene Name
THAP1
NCBI Description
The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.
Research Articles on THAP1
Similar Products
Product Notes
The THAP1 thap1 (Catalog #AAA204170) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's THAP1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the THAP1 thap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFK PTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAI GLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV EKLRKKLKTA QQRCRRQERQ LEKLKEVVHF QKEKDDVSER GYVILPNDYF EIVEVPA. It is sometimes possible for the material contained within the vial of "THAP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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