SDS-Page
(Figure. SDS-PAGE)
Transforming Growth Factor Beta 2 Active Protein | TGFb2 active protein
Active Transforming Growth Factor Beta 2 (TGFb2)
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Western Blot (WB)
(Figure. Western Blot)
Western Blot (WB)
(Figure. Western Blot)
Biological Activity Testing
(Transforming growth factor-beta 2 (TGF-2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Besides, Vitronectin (VTN) has been identified as an interactor of TGF-2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGF-2 and recombinant rat VTN. Briefly, TGF-2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to VTN-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TGF-2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-2 and VTN was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of TGF-2 with VTN.)
Biological Activity Testing
(Transforming growth factor-beta 2 (TGF-2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Besides, Vitronectin (VTN) has been identified as an interactor of TGF-2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGF-2 and recombinant rat VTN. Briefly, TGF-2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to VTN-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TGF-2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-2 and VTN was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of TGF-2 with VTN.)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 12kDa
NCBI Description
binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]
Uniprot Description
TGFB2: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Belongs to the TGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: axon; basement membrane; cell soma; cell surface; cytoplasm; endosome; extracellular matrix; extracellular region; extracellular space; secretory granule; trans-Golgi network
Molecular Function: beta-amyloid binding; cytokine activity; protein heterodimerization activity; protein homodimerization activity; protein N-terminus binding; punt binding; receptor binding; receptor signaling protein serine/threonine kinase activity; transforming growth factor beta receptor binding
Biological Process: activation of protein kinase activity; aging; axon guidance; blood vessel development; blood vessel remodeling; cardiac muscle cell proliferation; cardioblast differentiation; cartilage condensation; cell cycle arrest; cell death; cell development; cell migration; cell morphogenesis; collagen fibril organization; dopamine biosynthetic process; embryonic gut development; embryonic limb morphogenesis; embryonic neurocranium morphogenesis; epithelial to mesenchymal transition; extracellular matrix organization and biogenesis; eye development; female pregnancy; glial cell migration; gut development; hair follicle development; hair follicle morphogenesis; heart development; heart morphogenesis; hemopoiesis; hindbrain development; inner ear development; intercellular junction assembly and maintenance; kidney development; lung development; male gonad development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of immune response; negative regulation of Ras protein signal transduction; negative regulation of release of sequestered calcium ion into cytosol; neural tube closure; neuron development; neuron fate commitment; neutrophil chemotaxis; palate development; pancreas development; positive regulation of apoptosis; positive regulation of cardioblast differentiation; positive regulation of catagen; positive regulation of cell adhesion mediated by integrin; positive regulation of cell cycle; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of immune response; positive regulation of integrin biosynthetic process; positive regulation of neuron apoptosis; positive regulation of ossification; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of apoptosis; regulation of cell growth; regulation of cell proliferation; regulation of MAPKKK cascade; regulation of transforming growth factor-beta2 production; response to cold; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to estrogen stimulus; response to hypoxia; response to organic nitrogen; response to progesterone stimulus; response to radiation; response to retinoic acid; response to vitamin D; response to wounding; salivary gland morphogenesis; skeletal development; skeletal muscle development; SMAD protein nuclear translocation; somatic stem cell division; thyroid gland development; transforming growth factor beta receptor signaling pathway; uterus development; wound healing
Research Articles on TGFb2
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Product Notes
The TGFb2 tgfb2 (Catalog #AAA2122300) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala331~Ser442. AAA Biotech's Transforming Growth Factor Beta 2 can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the TGFb2 tgfb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor Beta 2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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