Typical Testing Data/Standard Curve (for reference only)
Porcine Transferrin Receptor ELISA Kit | TFR elisa kit
Porcine Transferrin Receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This TFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TFR. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY TFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFR antibody and an TFR-HRP conjugate. The assay sample and buffer are incubated together with TFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFR concentration since TFR from samples and TFR-HRP conjugate compete for the anti-TFR antibody binding site. Since the number of sites is limited, as more sites are occupied by TFR from the sample, fewer sites are left to bind TFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
Protein type: Membrane protein, integral; Cell surface; Receptor, misc.
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; vesicle; endosome; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding
Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; regulation of cell growth; osteoclast differentiation; transmembrane transport; regulation of cell proliferation
Research Articles on TFR
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Product Notes
The Porcine TFR tfrc (Catalog #AAA738334) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738334 ELISA Kit recognizes Porcine TFR. It is sometimes possible for the material contained within the vial of "Transferrin Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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