TFPI2 recombinant protein

Recombinant Human TFPI2 Protein (His tag)

Cat. Num. AAA2546191

• Gene Names: TFPI2; PP5; REF1; TFPI-2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TFPI2; Recombinant Human TFPI2 Protein (His tag); FLJ21164; PP5; REF1; TFPI-2; TFPI2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 235

• Application Notes: The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Asp 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TFPI2 recombinant protein

Background: Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.

Description: A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 5730091
• NCBI GeneID: 7980
• NCBI Accession #: NP_006519.1
• NCBI GenBank Nucleotide #: NM_006528.3
• UniProt Accession #: P48307; Q66ME8; Q8NAK6; Q9UC86
• Molecular Weight: 25,796 Da
• NCBI Official Full Name: tissue factor pathway inhibitor 2 isoform 1
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor 2
• NCBI Official Symbol: TFPI2
• NCBI Official Synonym Symbols: PP5; REF1; TFPI-2
• NCBI Protein Information: tissue factor pathway inhibitor 2
• UniProt Protein Name: Tissue factor pathway inhibitor 2
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI2
• UniProt Synonym Gene Names: TFPI-2; PP5

NCBI Description

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular matrix; nucleus; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent