Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit | TFPI2 elisa kit

Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit

Cat. Num. AAA36473

• Gene Names: TFPI2; PP5; REF1; TFPI-2
• Reactivity: Mouse
• Synonyms: Tissue Factor Pathway Inhibitor 2; Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit; TFPI2 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse TFPI2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 25 ng/ml – 800 ng/ml.
• Sensitivity: 2.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TFPI2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TFPI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TFPI2 and TFPI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TFPI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TFPI2 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TFPI2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TFPI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TFPI2 and TFPI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TFPI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 401871061
• NCBI GeneID: 7980
• NCBI Accession #: NP_001257933.1
• NCBI GenBank Nucleotide #: NM_001271004.1
• UniProt Accession #: P48307; Q66ME8; Q9UC86
• Molecular Weight: 26,934 Da
• NCBI Official Full Name: tissue factor pathway inhibitor 2 isoform 3
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor 2
• NCBI Official Symbol: TFPI2
• NCBI Official Synonym Symbols: PP5; REF1; TFPI-2
• NCBI Protein Information: tissue factor pathway inhibitor 2; placental protein 5; retinal pigment epithelium cell factor 1
• UniProt Protein Name: Tissue factor pathway inhibitor 2
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI2
• UniProt Synonym Gene Names: TFPI-2; PP5
• UniProt Entry Name: TFPI2_HUMAN

NCBI Description

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent

Biological Process: blood coagulation