Porcine Trefoil Factor 3, Intestinal ELISA Kit | TFF3 elisa kit

Porcine Trefoil Factor 3, Intestinal ELISA Kit

Cat. Num. AAA19520

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Porcine
• Synonyms: Trefoil Factor 3; Intestinal; Porcine Trefoil Factor 3; Intestinal ELISA Kit; TFF3 elisa kit

• Reactivity: Porcine
• Specificity: This kit recognizes recombinant and natural Porcine TFF3, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 62.5 ng/ml ~ 2000 ng/ml.
• Sensitivity: 10 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Porcine TFF3 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Porcine TFF3 concentration. The concentration of Porcine TFF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFF3 elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 281485608
• NCBI GeneID: 7033
• NCBI Accession #: NP_003217.3
• NCBI GenBank Nucleotide #: NM_003226.3
• UniProt Accession #: Q07654; Q96NX0; Q9UDA5; E9PBB5
• Molecular Weight: 8,641 Da
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3 (intestinal)
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3; polypeptide P1.B
• UniProt Protein Name: Trefoil factor 3
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI; hITF; hP1.B
• UniProt Entry Name: TFF3_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response