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SDS-Page

TFF1 recombinant protein

TFF1, 25-84aa, Human, His tag, E Coli

Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TFF1; 25-84aa; Human; His tag; E Coli; Trefoil factor 1; pS2; BCEI; HPS2; HP1.A; pNR-2; TFF1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MEAQTETCTV APRERQNCGF PGVTPSQCAN KGCCFDDTVR GVPWCFYPNT IDVPPEEECE F
Sequence Length
84
Applicable Applications for TFF1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for TFF1 recombinant protein
TFF1 (Trefoil factor 1) is member of trefoil family, characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. TFF1 has also been detected in a number of carcinomas including cancers of the breast, pancreas and stomach. Recombinant human TFF1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TFF1 recombinant protein
References
May FE., et al. (1997). J. Pathol. 183: 4-7.; Gott P, et al. (1997). Eur J Hum Genet 4 (6): 308-15.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9.0kDa (81aa), confirmed by MALDI-TOF
NCBI Official Full Name
trefoil factor 1
NCBI Official Synonym Full Names
trefoil factor 1
NCBI Official Symbol
TFF1
NCBI Official Synonym Symbols
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
NCBI Protein Information
trefoil factor 1
UniProt Protein Name
Trefoil factor 1
Protein Family
UniProt Gene Name
TFF1
UniProt Synonym Gene Names
BCEI; PS2; hP1.A

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Research Articles on TFF1

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Product Notes

The TFF1 tff1 (Catalog #AAA204253) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TFF1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TFF1 tff1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MEAQTETCTV APRERQNCGF PGVTPSQCAN KGCCFDDTVR GVPWCFYPNT IDVPPEEECE F. It is sometimes possible for the material contained within the vial of "TFF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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