Transcription Factor B2 Recombinant Protein | TFB2M recombinant protein
Recombinant Human Transcription Factor B2, Mitochondrial
Gene Names
TFB2M; Hkp1; mtTFB2
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Transcription Factor B2; Recombinant Human Transcription Factor B2; Mitochondrial; TFB2M Human; Mitochondrial Human Recombinant; Dimethyladenosine transferase 2 mitochondrial; Hepatitis C virus NS5A-transactivated protein 5; HCV NS5A-transactivated protein 5; Mitochondrial 12S rRNA dimethylase 2; Mitochondrial transcription factor B2; h-mtTFB; h-mtTFB2; hTFB2M; mtTFB2; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) dimethyltransferase 2; TFB2M; NS5ATP5; Hkp1; TFB2M recombinant protein
Host
E Coli
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
TFB2M protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGRFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN LGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R.
Sequence Length
396
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Related Product Information for TFB2M recombinant protein
Description: TFB2M Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 401 amino acids (20-396 a.a) and having a molecular mass of 45.8kDa.TFB2M is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: Transcription Factor B2, Mitochondrial (TFB2M) is an S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. In addition, TFB2M is essential for basal transcription of mitochondrial DNA, most likely via its interaction with POLRMT and TFAM. TFB2M promotes transcription independently of the methyltransferase activity. TFB2M like TFB1M, activates transcription of mitochondrial DNA more effectively, whilst having less methyltransferase activity.
Introduction: Transcription Factor B2, Mitochondrial (TFB2M) is an S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. In addition, TFB2M is essential for basal transcription of mitochondrial DNA, most likely via its interaction with POLRMT and TFAM. TFB2M promotes transcription independently of the methyltransferase activity. TFB2M like TFB1M, activates transcription of mitochondrial DNA more effectively, whilst having less methyltransferase activity.
Product Categories/Family for TFB2M recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,349 Da
NCBI Official Full Name
dimethyladenosine transferase 2, mitochondrial
NCBI Official Synonym Full Names
transcription factor B2, mitochondrial
NCBI Official Symbol
TFB2M
NCBI Official Synonym Symbols
Hkp1; mtTFB2
NCBI Protein Information
dimethyladenosine transferase 2, mitochondrial; HCV NS5A-transactivated protein 5; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2; h-mtTFB; h-mtTFB2; hTFB2M; hepatitis C virus NS5A-transactivated protein 5; mitochondrial 12S rRNA dimethylase 2; mitochondrial transcription factor B2
UniProt Protein Name
Dimethyladenosine transferase 2, mitochondrial
Protein Family
UniProt Gene Name
TFB2M
UniProt Synonym Gene Names
NS5ATP5; HCV NS5A-transactivated protein 5; h-mtTFB; h-mtTFB2; hTFB2M; mtTFB2
UniProt Entry Name
TFB2M_HUMAN
Uniprot Description
TFB2M: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
Protein type: EC 2.1.1.-; Methyltransferase; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: mitochondrial matrix
Molecular Function: rRNA (adenine-N6,N6-)-dimethyltransferase activity; transcription cofactor activity
Biological Process: mitochondrion organization and biogenesis; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; rRNA methylation; gene expression; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
Research Articles on TFB2M
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Product Notes
The TFB2M tfb2m (Catalog #AAA145466) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMAG RFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN LGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R.. It is sometimes possible for the material contained within the vial of "Transcription Factor B2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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