Sheep Transcription factor 20 ELISA Kit | TCF20 elisa kit

Sheep Transcription factor 20 ELISA Kit

Cat. Num. AAA737053

• Gene Names: TCF20; AR1; SPBP; TCF-20
• Reactivity: Sheep
• Synonyms: Transcription factor 20; Sheep Transcription factor 20 ELISA Kit; TCF20 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This TCF20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep TCF20. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCF20 elisa kit

Principle of the assay: TCF20 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TCF20. Standards or samples are then added to the microtiter plate wells and TCF20 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TCF20 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TCF20 are added to each well to “sandwich” the TCF20 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TCF20 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCF20 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TCF20 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 31652244
• NCBI GeneID: 6942
• NCBI Accession #: NP_005641.1
• NCBI GenBank Nucleotide #: NM_005650.2
• Molecular Weight: 209,654 Da
• NCBI Official Full Name: transcription factor 20 isoform 1
• NCBI Official Synonym Full Names: transcription factor 20 (AR1)
• NCBI Official Symbol: TCF20
• NCBI Official Synonym Symbols: AR1; SPBP; TCF-20
• NCBI Protein Information: transcription factor 20; nuclear factor SPBP; SPRE-binding protein; stromelysin-1 PDGF-responsive element-binding protein; stromelysin-1 platelet-derived growth factor-responsive element binding protein
• UniProt Protein Name: Transcription factor 20
• Protein Family: Transcription factor
• UniProt Gene Name: TCF20
• UniProt Synonym Gene Names: KIAA0292; SPBP; TCF-20; SPRE-binding protein
• UniProt Entry Name: TCF20_HUMAN

NCBI Description

This gene encodes a transcription factor that recognizes the platelet-derived growth factor-responsive element in the matrix metalloproteinase 3 promoter. The encoded protein is thought to be a transcriptional coactivator, enhancing the activity of transcription factors such as JUN and SP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

TCF20: a transcriptional activator. Binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator. Two alternatively spliced isoforms have been described. Isoform 1 is exclusively expressed in brain, heart and testis, and predominates in liver and kidney. Isoform 2 predominates in lung.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.3|22q13.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; zinc ion binding; transcription coactivator activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent