SDS-Page
(Recombinant TBP protein 10% SDS-PAGE Coomassie staining MW: 39 kDa Purity: >80%)
TBP recombinant protein
Recombinant TBP protein
Binding Assay conditions: 1 uM oligo dsDNA (DNA sequence: 5'-TCGTATAAAAGGC-3') was incubated with different concentrations of TBP protein in 10ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TBP protein 10% SDS-PAGE Coomassie staining MW: 39 kDa Purity: >80%)
SDS-Page
(Recombinant TBP protein 10% SDS-PAGE Coomassie staining MW: 39 kDa Purity: >80%)
Testing Data
(HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: TBP (also known as TATA box binding protein, GTF2D, transcription initiation factor TFIID TBP subunit, TATA-binding factor, TF2D, TFIID or SCA17). RNA polymerase II is an enzyme found in eukaryotic cells composed of 12 subunits that catalyzes transcription of DNA. Transcription consists of three phases: initiation, elongation and termination. Transcription initiation involves TFIID, which is composed of TATA-binding protein (TBP) and TBP-associated factors (TAFs), as part of the RNA polymerase II pre-initiation complex (PIC). TBP binds to the TATA box sequence, which is located upstream of the transcription start site in some eukaryotic gene promoters, and helps position RNA polymerase II. TBP contains a string of glutamines at the N-terminus which affect DNA binding and the rate of transcription complex formation. TBP is also associated with RNA polymerase I and RNA polymerase III.
NCBI and Uniprot Product Information
NCBI Description
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]
Research Articles on TBP
Similar Products
Product Notes
The TBP (Catalog #AAA389171) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. Application Notes: This protein is suitable for use in protein-protein interaction, in vitro transcription assay, binding assay. Binding Assay conditions: 1 uM oligo dsDNA (DNA sequence: 5'-TCGTATAAAAGGC-3') was incubated with different concentrations of TBP protein in 10ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. Researchers should empirically determine the suitability of the TBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
