Goat Thyroxine Binding Globulin ELISA Kit | TBG elisa kit

Goat Thyroxine Binding Globulin ELISA Kit

Cat. Num. AAA739732

• Gene Names: SERPINA7; TBG
• Reactivity: Goat
• Synonyms: Thyroxine Binding Globulin; Goat Thyroxine Binding Globulin ELISA Kit; TBG elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This TBG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TBG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TBG elisa kit

Principle of the Assay: TBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TBG antibody and an TBG-HRP conjugate. The assay sample and buffer are incubated together with TBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBG concentration since TBG from samples and TBG-HRP conjugate compete for the anti-TBG antibody binding site. Since the number of sites is limited, as more sites are occupied by TBG from the sample, fewer sites are left to bind TBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TBG elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 205277441
• NCBI GeneID: 6906
• NCBI Accession #: NP_000345.2
• NCBI GenBank Nucleotide #: NM_000354.5
• UniProt Accession #: P05543; D3DUX1
• Molecular Weight: 46,325 Da
• NCBI Official Full Name: thyroxine-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• NCBI Official Symbol: SERPINA7
• NCBI Official Synonym Symbols: TBG
• NCBI Protein Information: thyroxine-binding globulin; serpin A7; T4-binding globulin; thyroxin-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• UniProt Protein Name: Thyroxine-binding globulin
• UniProt Gene Name: SERPINA7
• UniProt Synonym Gene Names: TBG
• UniProt Entry Name: THBG_HUMAN

NCBI Description

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

Uniprot Description

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding

Biological Process: response to drug; response to peptide hormone stimulus; response to corticosterone stimulus; aging; post-embryonic development; response to vitamin A