Typical Testing Data/Standard Curve (for reference only)
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Human Thymosin beta 4 ELISA Kit | Tbeta4 elisa kit
Human Thymosin beta 4 ELISA Kit
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Reactivity
Human
Synonyms
Thymosin beta 4; Human Thymosin beta 4 ELISA Kit; Human Thymosin b 4 ELISA Kit; Tbeta4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TMbeta4. No significant cross-reactivity or interference between TMbeta4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMbeta4 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Sandwich
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for Tbeta4 elisa kit
Intended Uses: This TMbeta4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TMbeta4. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: TMbeta4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TMbeta4. Standards or samples are then added to the microtiter plate wells and TMbeta4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TMbeta4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TMbeta4 are added to each well to "sandwich" the TMbeta4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TMbeta4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMbeta4 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: TMbeta4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TMbeta4. Standards or samples are then added to the microtiter plate wells and TMbeta4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TMbeta4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TMbeta4 are added to each well to "sandwich" the TMbeta4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TMbeta4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMbeta4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Tbeta4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,053 Da
NCBI Official Full Name
thymosin beta-4
NCBI Official Synonym Full Names
thymosin beta 4, X-linked
NCBI Official Symbol
TMSB4X
NCBI Official Synonym Symbols
FX; TB4X; PTMB4; TMSB4
NCBI Protein Information
thymosin beta-4; t beta-4; prothymosin beta-4; thymosin, beta 4, X chromosome
UniProt Protein Name
Thymosin beta-4
UniProt Gene Name
TMSB4X
UniProt Synonym Gene Names
TB4X; THYB4; TMSB4; T beta-4
UniProt Entry Name
TYB4_HUMAN
NCBI Description
This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]
Uniprot Description
TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq21.3-q22
Cellular Component: cytoskeleton; extracellular region
Molecular Function: actin monomer binding; protein binding
Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation
Research Articles on Tbeta4
Similar Products
Product Notes
The Human Tbeta4 tmsb4x (Catalog #AAA726209) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726209 ELISA Kit recognizes Human Tbeta4. It is sometimes possible for the material contained within the vial of "Thymosin beta 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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