Taq Plus DNA Polymerase Recombinant Protein
Recombinant Taq Plus DNA Polymerase
Synonyms
Taq Plus DNA Polymerase; Recombinant Taq Plus DNA Polymerase; Taq Plus DNA; Taq Plus DNA Polymerase Recombinant; TaqPDNA; Taq Plus DNA Polymerase recombinant protein
Host
Recombinant E Coli contains Thermus aquaticus polymerase gene
Concentration
5U/ ul. (varies by lot)
10X Reaction Buffer
200 mM TrisHCI (pH 8.8)100 mM KCI100 mM (NH4)2SO420 mM Mg SO41% Triton X-1001 mg/ml bovine serum albumin (BSA).
Optimization of DNA Synthesis
It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus.
Reaction Conditions
DNA synthesis is performed in 100ml of mixture containing 20-200um dNTPs, 0.3-1um Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA.
Unit Defenition
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74 degree C.
Storage Buffer
20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.
Note
All reagents, including Taq Plus, should be mixed immediately before use.
Preparation and Storage
Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C.
Related Product Information for Taq Plus DNA Polymerase recombinant protein
Description: Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78 degree C and remains > 95% active following 1-hour incubation at 95 degree C.
Product Categories/Family for Taq Plus DNA Polymerase recombinant protein
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Product Notes
The Taq Plus DNA Polymerase (Catalog #AAA142450) is a Recombinant Protein produced from Recombinant E Coli contains Thermus aquaticus polymerase gene and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Taq Plus DNA Polymerase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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