TAP2 / Antigen Peptide Transporter 2 Recombinant Protein | TAP2 recombinant protein

Human TAP2 / Antigen Peptide Transporter 2 Recombinant Protein

• Gene Names: TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: TAP2 / Antigen Peptide Transporter 2; Human TAP2 / Antigen Peptide Transporter 2 Recombinant Protein; Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein; ABCB3; PSF2; RING11; Y1; TAP2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 686

• Applicable Applications for TAP2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Heterodimer of TAP1 and TAP2. Interacts with Epstein-Barr virus BLNF2a. Interacts with herpes simplex virus ICP47 (PubMed:7760936).
• Entry Function: Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 549044
• NCBI GeneID: 6891
• UniProt Accession #: Q03519
• NCBI Official Full Name: Antigen peptide transporter 2
• NCBI Official Synonym Full Names: transporter 2, ATP binding cassette subfamily B member
• NCBI Official Symbol: TAP2
• NCBI Official Synonym Symbols: APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
• NCBI Protein Information: antigen peptide transporter 2
• UniProt Protein Name: Antigen peptide transporter 2
• Protein Family: Protein
• UniProt Gene Name: TAP2
• UniProt Synonym Gene Names: ABCB3; PSF2; RING11; Y1; APT2; PSF-2
• UniProt Entry Name: TAP2_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]

Uniprot Description

TAP2: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Transports peptides across the endoplasmic reticulum membrane for assembly of major histocompatibility complex class I molecules. Two subunits, TAP1 and TAP2, are required for peptide transport. ATP hydrolysis is important for transport activity. Nascent MHC class I molecules associate with TAP via tapasin.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; TAP complex; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: tapasin binding; protein binding; peptide transporter activity; TAP1 binding; transporter activity; peptide antigen-transporting ATPase activity; ATP binding

Biological Process: intracellular transport of viral proteins in host cell; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I; cytosol to ER transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen transport; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Bare Lymphocyte Syndrome, Type I