Mouse Trypsinogen ELISA Kit | TAP elisa kit

Mouse Trypsinogen activation peptide (TAP) ELISA Kit

Cat. Num. AAA285523

• Gene Names: PRSS2; TRY2; TRY8; TRYP2
• Reactivity: Mouse
• Synonyms: Trypsinogen; Mouse Trypsinogen activation peptide (TAP) ELISA Kit; TAP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse TAP. No significant cross-reactivity or interference between Mouse TAP and analogues was observed.
• Sequence Length: 247

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for TAP elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TAP in samples. An antibody specific for TAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 521218
• NCBI GeneID: 5645
• Molecular Weight: 26,488 Da
• NCBI Official Full Name: trypsinogen
• NCBI Official Synonym Full Names: protease, serine 2
• NCBI Official Symbol: PRSS2
• NCBI Official Synonym Symbols: TRY2; TRY8; TRYP2
• NCBI Protein Information: trypsin-2
• UniProt Protein Name: Trypsin-2
• Protein Family: Trypsinogen-like protein
• UniProt Gene Name: PRSS2
• UniProt Synonym Gene Names: TRY2; TRYP2
• UniProt Entry Name: TRY2_HUMAN

NCBI Description

This gene belongs to the trypsin family of serine proteases and encodes anionic trypsinogen. It is part of a cluster of trypsinogen genes that are located within the T cell receptor beta locus. Enzymes of this family cleave peptide bonds that follow lysine or arginine residues. This protein is found at high levels in pancreatic juice and its upregulation is a characteristic feature of pancreatitis. This protein has also been found to activate pro-urokinase in ovarian tumors, suggesting a function in tumor invasion. In addition, this enzyme is able to cleave across the type II collagen triple helix in rheumatoid arthritis synovitis tissue, potentially participating in the degradation of type II collagen-rich cartilage matrix. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2015]

Uniprot Description

trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.

Protein type: Cell adhesion; Secreted, signal peptide; Calcium-binding; EC 3.4.21.4; Extracellular matrix; Secreted; Protease

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: calcium ion binding; protein binding; serine-type endopeptidase activity

Biological Process: collagen catabolic process; digestion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; innate immune response; positive regulation of cell adhesion; positive regulation of cell growth; proteolysis

Disease: Pancreatitis, Hereditary