Human transgelin ELISA Kit | TAGLN elisa kit

Human transgelin, TAGLN ELISA Kit

Cat. Num. AAA927405

• Gene Names: TAGLN; SM22; SMCC; TAGLN1; WS3-10
• Reactivity: Human
• Synonyms: transgelin; Human transgelin; TAGLN ELISA Kit; Human transgelin (TAGLN) ELISA kit; DKFZp686B01212; DKFZp686P11128; SM22; SMCC; TAGLN1; WS3-10; SM22-alpha; transgelin variant 2; TAGLN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TAGLN. No significant cross-reactivity or interference between Human TAGLN and analogues was observed.
• Sequence Length: 201

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAGLN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TAGLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAGLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 48255907
• NCBI GeneID: 6876
• NCBI Accession #: NP_001001522.1
• NCBI GenBank Nucleotide #: NM_001001522.1
• UniProt Accession #: Q01995; O15542
• Molecular Weight: 22,611 Da
• NCBI Official Full Name: transgelin
• NCBI Official Synonym Full Names: transgelin
• NCBI Official Symbol: TAGLN
• NCBI Official Synonym Symbols: SM22; SMCC; TAGLN1; WS3-10
• NCBI Protein Information: transgelin; SM22-alpha; transgelin variant 2; 22 kDa actin-binding protein; smooth muscle protein 22-alpha
• UniProt Protein Name: Transgelin
• Protein Family: Transgelin
• UniProt Gene Name: TAGLN
• UniProt Synonym Gene Names: SM22; WS3-10; SM22-alpha
• UniProt Entry Name: TAGL_HUMAN

NCBI Description

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: cytoplasm

Molecular Function: actin filament binding; protein binding

Biological Process: muscle development; epithelial cell differentiation