Transcription initiation factor TFIID subunit 14 (TAF14) Recombinant Protein | TAF14 recombinant protein

Recombinant Saccharomyces cerevisiae Transcription initiation factor TFIID subunit 14 (TAF14)

Cat. Num. AAA1020831

• Gene Names: TAF14; ANC1; SWP29; TAF30; TFG3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transcription initiation factor TFIID subunit 14 (TAF14); Recombinant Saccharomyces cerevisiae Transcription initiation factor TFIID subunit 14 (TAF14); TAF14 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-244, full length protein
• Sequence: MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE
• Sequence Length: 244

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 6325128
• NCBI GeneID: 855974
• NCBI Accession #: NP_015196.1
• NCBI GenBank Nucleotide #: NM_001183943.1
• UniProt Accession #: P35189; Q02460; D6W3N8
• Molecular Weight: 27,440 Da
• NCBI Official Full Name: TATA-binding protein-associated factor TAF14
• NCBI Official Symbol: TAF14
• NCBI Official Synonym Symbols: ANC1; SWP29; TAF30; TFG3
• NCBI Protein Information: TATA-binding protein-associated factor TAF14
• UniProt Protein Name: Transcription initiation factor TFIID subunit 14
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF14
• UniProt Synonym Gene Names: ANC1; CST10; SWP29; TAF30; TFG3

Uniprot Description

Functions as a component of the DNA-binding general transcription factor complex TFIID, the RNA polymerase II associated general transcription factor complex TFIIF, and the chromatin-remodeling complex SWI/SNF (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449). Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449). TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449). TFIIF is essential for the initiation of transcription by RNA polymerase II (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449). TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449). TAF14 acts as a chromatin reader that specifically recognizes and binds histones that are acylated (PubMed:26341557, PubMed:27089029). Recognizes and binds histone H3 acetylated or crotonylated at 'Lys-9' (H3K9ac and H3K9cr, respectively) (PubMed:26341557, PubMed:27089029). Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes (PubMed:12672490). It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors (PubMed:12672490). Component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3 (PubMed:17157260). In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation (PubMed:17157260).