SDS-Page
(Recombinant TAF1 (1522-1656) protein gel. TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.3 kDa Purity: ? 90%)
TAF1 recombinant protein
Recombinant TAF1 (1522-1656) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TAF1 (1522-1656) protein gel. TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.3 kDa Purity: ? 90%)
SDS-Page
(Recombinant TAF1 (1522-1656) protein gel. TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.3 kDa Purity: ? 90%)
Testing Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Testing Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Testing Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Testing Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Background: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.
NCBI and Uniprot Product Information
NCBI Description
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq, Aug 2009]
Uniprot Description
TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.1.48; DNA-binding; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, atypical; Transcription, coactivator/corepressor; ATYPICAL group; TAF1 family
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: nucleoplasm; transcription factor TFIID complex
Molecular Function: protein serine/threonine kinase activity; protein binding; histone acetyltransferase activity; p53 binding; sequence-specific DNA binding; TATA-binding protein binding; transcription coactivator activity; transcription factor binding; ATP binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein amino acid autophosphorylation; transcription initiation; peptidyl-threonine phosphorylation; transcriptional preinitiation complex assembly; cell cycle; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; histone acetylation; response to DNA damage stimulus
Disease: Dystonia 3, Torsion, X-linked
Research Articles on TAF1
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Product Notes
The TAF1 taf1 (Catalog #AAA388153) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TAF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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