Typical Testing Data/Standard Curve (for reference only)
Rat Tachykinin Receptor 3 ELISA Kit | TACR3 elisa kit
Rat Tachykinin Receptor 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: T3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-T3 antibody and an T3-HRP conjugate. The assay sample and buffer are incubated together with T3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the T3 concentration since T3 from samples and T3-HRP conjugate compete for the anti-T3 antibody binding site. Since the number of sites is limited, as more sites are occupied by T3 from the sample, fewer sites are left to bind T3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neurokinin 3, also referred to as neurokinin B. [provided by RefSeq, Jul 2008]
Uniprot Description
TACR3: This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 4q25
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nucleus
Molecular Function: protein binding; tachykinin receptor activity
Biological Process: tachykinin signaling pathway; hyperosmotic salinity response; regulation of dopamine metabolic process; response to morphine; positive regulation of heart rate; response to cocaine; positive regulation of blood pressure; response to estradiol stimulus; aging
Disease: Hypogonadotropic Hypogonadism 11 With Or Without Anosmia
Research Articles on TACR3
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Product Notes
The Rat TACR3 tacr3 (Catalog #AAA720166) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720166 ELISA Kit recognizes Rat TACR3. It is sometimes possible for the material contained within the vial of "Tachykinin Receptor 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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