Neurokinin B (NKB) Recombinant Protein | NKB recombinant protein

Recombinant Neurokinin B (NKB)

Cat. Num. AAA2018392

• Gene Names: TAC3; NKB; HH10; NKNB; PRO1155; ZNEUROK1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Neurokinin B (NKB); Recombinant Neurokinin B (NKB); NKB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CKEPQEEV VPGGGRSKRD PDLYQLLQRL FKSHSSLEGL LKALSQASTD PKESTSPEKR DMHDFFVGLM GKRSVQPDSPTDVNQENVPS FGILKYPPRA E
• Sequence Length: 103

• Applicable Applications for NKB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Cys23~Glu121 (Accession # Q9UHF0) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 295849290
• NCBI GeneID: 6866
• NCBI Accession #: NP_001171525.1
• NCBI GenBank Nucleotide #: NM_001178054.1
• UniProt Accession #: Q9UHF0; Q6IAG2; Q71BC6; Q71BC9
• Molecular Weight: 14.9kDa
• NCBI Official Full Name: tachykinin-3 isoform 2 preproprotein
• NCBI Official Synonym Full Names: tachykinin 3
• NCBI Official Symbol: TAC3
• NCBI Official Synonym Symbols: NKB; HH10; NKNB; PRO1155; ZNEUROK1
• NCBI Protein Information: tachykinin-3; neuromedin K; gamma tachykinin 3; preprotachykinin B; preprotachykinin-B
• UniProt Protein Name: Tachykinin-3
• UniProt Gene Name: TAC3
• UniProt Synonym Gene Names: NKNB; NKB
• UniProt Entry Name: TKNK_HUMAN

NCBI Description

This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded protein is primarily expressed in the central and peripheral nervous system and functions as a neurotransmitter. This protein is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; receptor binding

Biological Process: tachykinin signaling pathway; neuropeptide signaling pathway; female pregnancy

Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia