XBP1 sirna

XBP1 siRNA (Rat)

Cat. Num. AAA8228819

• Gene Names: Xbp1; HTF
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: XBP1; XBP1 siRNA (Rat); HTF; X-box-binding protein 1; Hepatocarcinogenesis-related transcription factor; XBP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: XBP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 267

• Applicable Applications for XBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat XBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for XBP1 sirna

siRNA to inhibit XBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51948392
• NCBI GeneID: 289754
• NCBI Accession #: NP_001004210.1
• NCBI GenBank Nucleotide #: NM_001004210.2
• UniProt Accession #: Q9R1S4
• Molecular Weight: 29,665 Da
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(U)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: Xbp1
• NCBI Official Synonym Symbols: HTF
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: Xbp1
• UniProt Synonym Gene Names: HTF
• UniProt Entry Name: XBP1_RAT

NCBI Description

transcription factor; regulates MHC class II genes by binding to an X-box promoter element; agents that cause the unfolded protein response upregulate transcription [RGD, Feb 2006]

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: DNA-binding; Transcription factor

Cellular Component: endoplasmic reticulum; cell; cytoplasm; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: DNA binding; protease binding; sequence-specific DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; histone deacetylase binding; protein kinase binding; transcription factor activity

Biological Process: ubiquitin-dependent protein catabolic process; transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; cellular response to glucose starvation; regulation of transcription, DNA-dependent; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; response to electrical stimulus; cell growth; positive regulation of autophagy; positive regulation of TOR signaling pathway; response to drug; positive regulation of histone methylation; protein destabilization; epithelial cell maturation; unfolded protein response; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; regulation of transcription from RNA polymerase II promoter; positive regulation of immunoglobulin production; cholesterol homeostasis; positive regulation of T cell differentiation; cellular response to insulin stimulus; endothelial cell proliferation; fatty acid homeostasis; positive regulation of B cell differentiation; positive regulation of fat cell differentiation; neuron development; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; sterol homeostasis; negative regulation of apoptosis