VAPB sirna

VAPB siRNA (Rat)

Cat. Num. AAA8226217

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAPB; VAPB siRNA (Rat); Vesicle-associated membrane protein-associated protein B; VAMP-B; VAMP-associated protein B; VAP-B; VAPB sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: VAPB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 243

• Applicable Applications for VAPB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VAPB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAPB sirna

siRNA to inhibit VAPB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11177880
• NCBI GeneID: 60431
• NCBI Accession #: NP_068619.1
• NCBI GenBank Nucleotide #: NM_021847.3
• UniProt Accession #: Q9Z269
• Molecular Weight: 26,916 Da
• NCBI Official Full Name: vesicle-associated membrane protein-associated protein B
• NCBI Official Synonym Full Names: VAMP (vesicle-associated membrane protein)-associated protein B and C
• NCBI Official Symbol: Vapb
• NCBI Protein Information: vesicle-associated membrane protein-associated protein B
• UniProt Protein Name: Vesicle-associated membrane protein-associated protein B
• Protein Family: Virulence-associated protein
• UniProt Gene Name: Vapb
• UniProt Synonym Gene Names: VAMP-B; VAMP-associated protein B; VAP-B
• UniProt Entry Name: VAPB_RAT

NCBI Description

may play a role in exocytosis of neurotransmitters [RGD, Feb 2006]

Uniprot Description

VAPB: Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. Defects in VAPB are a cause of spinal muscular atrophy proximal adult autosomal dominant (SMAPAD); also called late-onset spinal muscular atrophy Finkel type. A form of spinal muscular atrophy, a neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAPAD is characterized by proximal muscle weakness that begins in the lower limbs and then progresses to upper limbs, onset in late adulthood (after third decade) and a benign course. Most of the patients remain ambulatory 10 to 40 years after clinical onset. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; protein homodimerization activity; FFAT motif binding; protein heterodimerization activity; microtubule binding; beta-tubulin binding

Biological Process: cellular calcium ion homeostasis; endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; unfolded protein response, activation of signaling protein activity; positive regulation of viral genome replication; unfolded protein response; virus-host interaction; negative regulation of viral protein levels in host cell