VAMP8 sirna

VAMP8 siRNA (Rat)

Cat. Num. AAA8228169

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAMP8; VAMP8 siRNA (Rat); Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; EDB; VAMP8 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: VAMP8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 100

• Applicable Applications for VAMP8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VAMP8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAMP8 sirna

siRNA to inhibit VAMP8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929182
• NCBI GeneID: 83730
• NCBI Accession #: NP_114015.1
• NCBI GenBank Nucleotide #: NM_031827.1
• UniProt Accession #: Q9WUF4
• Molecular Weight: 11,320 Da
• NCBI Official Full Name: vesicle-associated membrane protein 8
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 8
• NCBI Official Symbol: Vamp8
• NCBI Protein Information: vesicle-associated membrane protein 8
• UniProt Protein Name: Vesicle-associated membrane protein 8
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: Vamp8
• UniProt Synonym Gene Names: EDB
• UniProt Entry Name: VAMP8_RAT

NCBI Description

may mediate exocytosis of synaptic vesicles in neurons [RGD, Feb 2006]

Uniprot Description

VAMP8: SNAREs, Soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes

Protein type: Membrane protein, integral; Vesicle

Cellular Component: cell surface; mitochondrion; late endosome membrane; secretory granule membrane; lysosomal membrane; integral to membrane; cytosol; secretory granule; SNARE complex; recycling endosome; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; vesicle

Molecular Function: SNAP receptor activity; protein binding; chloride channel inhibitor activity; syntaxin binding

Biological Process: vesicle fusion; vesicle-mediated transport; entry of virus into host cell; protein transport; exocytosis; autophagic vacuole fusion; neutrophil degranulation; regulation of endocytosis; eosinophil degranulation; protein complex assembly; endocytosis