VAMP1 sirna

VAMP1 siRNA (Rat)

Cat. Num. AAA8202564

• Gene Names: Vamp1; Syb1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAMP1; VAMP1 siRNA (Rat); SYB1; Vesicle-associated membrane protein 1; VAMP-1; Synaptobrevin-1; VAMP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: VAMP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 118

• Applicable Applications for VAMP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VAMP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAMP1 sirna

siRNA to inhibit VAMP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 76443677
• NCBI GeneID: 25624
• NCBI Accession #: NP_037222.2
• NCBI GenBank Nucleotide #: NM_013090.2
• UniProt Accession #: Q63666; O09025; Q56A22; Q8CH14; A6YSN3
• Molecular Weight: 13,940 Da
• NCBI Official Full Name: vesicle-associated membrane protein 1
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 1
• NCBI Official Symbol: Vamp1
• NCBI Official Synonym Symbols: Syb1
• NCBI Protein Information: vesicle-associated membrane protein 1
• UniProt Protein Name: Vesicle-associated membrane protein 1
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: Vamp1
• UniProt Synonym Gene Names: Syb1; VAMP-1
• UniProt Entry Name: VAMP1_RAT

NCBI Description

plays a role in protein transport to the plasma membrane [RGD, Feb 2006]

Uniprot Description

VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: SNARE complex; mitochondrial outer membrane; synaptic vesicle; cell surface; cytoplasmic vesicle membrane; synaptic vesicle membrane; mitochondrion; integral to membrane; terminal button; cytosol; cell junction

Molecular Function: SNAP receptor activity; SNARE binding; protein binding

Biological Process: vesicle-mediated transport; vesicle fusion; intracellular protein transport; exocytosis; neurotransmitter secretion