USF2 sirna

USF2 siRNA (Rat)

Cat. Num. AAA8211745

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USF2; USF2 siRNA (Rat); Upstream stimulatory factor 2; Major late transcription factor 2; Upstream transcription factor 2; USF2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: USF2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 346

• Applicable Applications for USF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat USF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USF2 sirna

siRNA to inhibit USF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13592125
• NCBI GeneID: 81817
• NCBI Accession #: NP_112401.1
• NCBI GenBank Nucleotide #: NM_031139.1
• UniProt Accession #: Q63665; Q76MJ1; Q76MU2; Q9EQX1; Q9EQX2; Q9EQX3
• Molecular Weight: 2,145 Da
• NCBI Official Full Name: upstream stimulatory factor 2
• NCBI Official Synonym Full Names: upstream transcription factor 2, c-fos interacting
• NCBI Official Symbol: Usf2
• NCBI Protein Information: upstream stimulatory factor 2
• UniProt Protein Name: Upstream stimulatory factor 2
• Protein Family: Upstream stimulatory factor
• UniProt Gene Name: Usf2
• UniProt Entry Name: USF2_RAT

NCBI Description

ubiquitous transcription factor; may play a role in hormonal and dietary regulations [RGD, Feb 2006]

Uniprot Description

USF2: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; lactation; positive regulation of transcription, DNA-dependent; lipid homeostasis; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter