UGT2B17 sirna

UGT2B17 siRNA (Rat)

Cat. Num. AAA8212958

• Gene Names: Ugt2b17; Udpgt; Rlug38; Ugt2b3; Ugt2b5; Udpgtr-3; UDPGT 2B5
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UGT2B17; UGT2B17 siRNA (Rat); UGT2B3; UGT2B5; UDP-glucuronosyltransferase 2B17; UDPGT 2B17; 17-beta-hydroxysteroid-specific UDPGT; RLUG38; Testosterone. dihydrotestosterone. and beta-estradiol-specific UDPGT; UDP-glucuronosyltransferase 2B5; UDPGT 2B5; UDPGTr-3; UGT2B17 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: UGT2B17 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 530

• Applicable Applications for UGT2B17 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat UGT2B17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UGT2B17 sirna

siRNA to inhibit UGT2B17 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543923
• NCBI GeneID: 266685
• NCBI Accession #: NP_695226.2
• NCBI GenBank Nucleotide #: NM_153314.2
• UniProt Accession #: P08542; P16915
• Molecular Weight: 60,525 Da
• NCBI Official Full Name: UDP-glucuronosyltransferase 2B17
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase 2 family, polypeptide B15
• NCBI Official Symbol: Ugt2b17
• NCBI Official Synonym Symbols: Udpgt; Rlug38; Ugt2b3; Ugt2b5; Udpgtr-3; UDPGT 2B5
• NCBI Protein Information: UDP-glucuronosyltransferase 2B17
• UniProt Protein Name: UDP-glucuronosyltransferase 2B17
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: Ugt2b17
• UniProt Synonym Gene Names: Ugt2b3; Ugt2b5; UDPGT 2B17; UDPGT 2B5
• UniProt Entry Name: UDB17_RAT

NCBI Description

enzyme which glucuronidates testosterone, dihydrotestosterone, and beta-estradiol [RGD, Feb 2006]

Uniprot Description

UGT2B17: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. The major substrates of this isozyme are eugenol > 4-methylumbelliferone > dihydrotestosterone (DHT) > androstane-3-alpha,17-beta-diol (3-alpha-diol) > testosterone > androsterone (ADT). Belongs to the UDP-glycosyltransferase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Carbohydrate Metabolism - pentose and glucuronate interconversions; Mitochondrial; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.4.1.17; Carbohydrate Metabolism - starch and sucrose; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Carbohydrate Metabolism - ascorbate and aldarate; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: transferase activity, transferring hexosyl groups; glucuronosyltransferase activity

Biological Process: flavonoid biosynthetic process; metabolic process; response to lipopolysaccharide