SYNJ1 sirna

SYNJ1 siRNA (Rat)

Cat. Num. AAA8223960

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYNJ1; SYNJ1 siRNA (Rat); Synaptojanin-1; Synaptic inositol 1.4.5-trisphosphate 5-phosphatase 1; SYNJ1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SYNJ1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1292

• Applicable Applications for SYNJ1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SYNJ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYNJ1 sirna

siRNA to inhibit SYNJ1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209870039
• NCBI GeneID: 85238
• NCBI Accession #: NP_445928.2
• NCBI GenBank Nucleotide #: NM_053476.2
• UniProt Accession #: Q62910; O89092; Q62911; Q810Z8
• Molecular Weight: 126,166 Da
• NCBI Official Full Name: synaptojanin-1
• NCBI Official Synonym Full Names: synaptojanin 1
• NCBI Official Symbol: Synj1
• NCBI Protein Information: synaptojanin-1
• UniProt Protein Name: Synaptojanin-1
• Protein Family: Synaptojanin
• UniProt Gene Name: Synj1
• UniProt Entry Name: SYNJ1_RAT

NCBI Description

has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

Uniprot Description

SYNJ1: Inositol 5-phosphatase which has a role in clathrin- mediated endocytosis. Belongs to the synaptojanin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; Vesicle; Motility/polarity/chemotaxis; EC 3.1.3.36; Phosphatase, lipid

Cellular Component: neuron projection; protein complex; membrane coat; cell; cytoplasm

Molecular Function: protein C-terminus binding; protein binding; nucleic acid binding; RNA binding; phosphoinositide 5-phosphatase activity; protein complex binding; nucleotide binding; SH3 domain binding; phosphoric ester hydrolase activity

Biological Process: response to retinoic acid; positive regulation of gliogenesis; synaptic vesicle transport; synaptic vesicle uncoating; learning; positive regulation of receptor-mediated endocytosis; neurotransmitter transport; response to cytokine stimulus; inositol phosphate dephosphorylation; synaptic vesicle endocytosis; phosphoinositide dephosphorylation; brain development; phosphatidylinositol metabolic process; synaptic vesicle priming