SUB1 sirna

SUB1 siRNA (Rat)

Cat. Num. AAA8210811

• Gene Names: Sub1; Rpo2tc1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SUB1; SUB1 siRNA (Rat); PC4; RPO2TC1; Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; SUB1 homolog; p14; SUB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SUB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 127

• Applicable Applications for SUB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SUB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SUB1 sirna

siRNA to inhibit SUB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 57526804
• NCBI GeneID: 192269
• NCBI Accession #: NP_001009618.1
• NCBI GenBank Nucleotide #: NM_001009618.1
• UniProt Accession #: Q63396; Q5M805
• Molecular Weight: 14,441 Da
• NCBI Official Full Name: activated RNA polymerase II transcriptional coactivator p15
• NCBI Official Synonym Full Names: SUB1 homolog (S. cerevisiae)
• NCBI Official Symbol: Sub1
• NCBI Official Synonym Symbols: Rpo2tc1
• NCBI Protein Information: activated RNA polymerase II transcriptional coactivator p15
• UniProt Protein Name: Activated RNA polymerase II transcriptional coactivator p15
• Protein Family: Subtilisin
• UniProt Gene Name: Sub1
• UniProt Synonym Gene Names: Pc4; Rpo2tc1; PC4
• UniProt Entry Name: TCP4_RAT

NCBI Description

expressed in 12 day embryo and in a chemically induced pancreatic B-cell tumor; not expressed in normal differentiated cells [RGD, Feb 2006]

Uniprot Description

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function: transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter