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STEAP3 sirna

STEAP3 siRNA (Rat)

Reactivity
Rat
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
STEAP3; STEAP3 siRNA (Rat); Metalloreductase STEAP3; Six-transmembrane epithelial antigen of prostate 3; pHyde; STEAP3 sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Rat
Specificity
STEAP3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
488
Applicable Applications for STEAP3 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat STEAP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for STEAP3 sirna
siRNA to inhibit STEAP3 expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,674 Da
NCBI Official Full Name
metalloreductase STEAP3
NCBI Official Synonym Full Names
STEAP family member 3, metalloreductase
NCBI Official Symbol
Steap3
NCBI Protein Information
metalloreductase STEAP3
UniProt Protein Name
Metalloreductase STEAP3
Protein Family
UniProt Gene Name
Steap3
UniProt Entry Name
STEA3_RAT

NCBI Description

induces apoptosis; inhibits prostate tumor growth in some cell lines [RGD, Feb 2006]

Uniprot Description

STEAP3: a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor. Contains a highly-conserved tyrosine-based motif that has been shown to target transmembrane proteins, including LAMP-1, LAMP-2, the TfR, and SLC11A1 to endosomes, lysosomes, and lysosome-related organelles. Facilitates the exosomal secretion of proteins such as TCTP. Interacts with BNIP3L, MYT1 and TPT1. Four human isoforms are produced by alternative splicing. Isoform 4 is also known as pHyde II.

Protein type: Membrane protein, integral; Transporter, iron; Oxidoreductase; Membrane protein, multi-pass; EC 1.16.1.-; Transporter, ion channel; Transporter

Cellular Component: multivesicular body; cell; plasma membrane; integral to membrane; endosome membrane; endosome

Molecular Function: metal ion binding; ferric-chelate reductase activity; cupric reductase activity

Biological Process: iron ion homeostasis; apoptosis; positive regulation of apoptosis; protein secretion; copper ion import; iron ion transport

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Product Notes

The STEAP3 steap3 (Catalog #AAA8222769) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The STEAP3 siRNA (Rat) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STEAP3 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the STEAP3 steap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STEAP3, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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