SPTBN2 sirna

SPTBN2 siRNA (Rat)

Cat. Num. AAA8231033

• Gene Names: Sptbn2; Spnb3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPTBN2; SPTBN2 siRNA (Rat); SPNB3; Spectrin beta chain. non-erythrocytic 2; Beta SpIII sigma 1; Beta-III spectrin; Glutamate transporter EAAT4-associated protein 41; SPNB-3; Spectrin-like protein GTRAP41; SPTBN2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SPTBN2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,388

• Applicable Applications for SPTBN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SPTBN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPTBN2 sirna

siRNA to inhibit SPTBN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9507135
• NCBI GeneID: 29211
• NCBI Accession #: NP_062040.1
• NCBI GenBank Nucleotide #: NM_019167.1
• UniProt Accession #: Q9QWN8; O88197; Q9ES68
• Molecular Weight: 271,064 Da
• NCBI Official Full Name: spectrin beta chain, non-erythrocytic 2
• NCBI Official Synonym Full Names: spectrin, beta, non-erythrocytic 2
• NCBI Official Symbol: Sptbn2
• NCBI Official Synonym Symbols: Spnb3
• NCBI Protein Information: spectrin beta chain, non-erythrocytic 2
• UniProt Protein Name: Spectrin beta chain, non-erythrocytic 2
• Protein Family: Spectrin
• UniProt Gene Name: Sptbn2
• UniProt Synonym Gene Names: Spnb3
• UniProt Entry Name: SPTN2_RAT

NCBI Description

may modulate the structural integrity of the Golgi apparatus; binds preferentially to Arp1 rather than actin to mediate the direct interaction of dynein and dynactin with vesicles [RGD, Feb 2006]

Uniprot Description

SPTBN2: Probably plays an important role in neuronal membrane skeleton. Defects in SPTBN2 are the cause of spinocerebellar ataxia type 5 (SCA5). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA5 is an autosomal dominant cerebellar ataxia (ADCA). It is a slowly progressive disorder with variable age at onset, ranging between 10 and 50 years. Belongs to the spectrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: spectrin; extracellular space; nuclear matrix; cell soma; apical plasma membrane; cytoplasm; cytosol; cell junction

Molecular Function: structural constituent of cytoskeleton; phospholipid binding; actin binding

Biological Process: vesicle-mediated transport; synaptic vesicle exocytosis; synaptogenesis; actin filament capping; multicellular organism growth; adult behavior; cerebellar Purkinje cell layer morphogenesis; regulation of calcium ion-dependent exocytosis; synaptic vesicle docking during exocytosis