SLC34A1 sirna

SLC34A1 siRNA (Rat)

Cat. Num. AAA8215128

• Gene Names: Slc34a1; Npt2; NaPi-2; NaPi2A; Slc17a2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC34A1; SLC34A1 siRNA (Rat); SLC17A2; Sodium-dependent phosphate transport protein 2A; Sodium-phosphate transport protein 2A; Na(+)-dependent phosphate cotransporter 2A; Sodium/phosphate cotransporter 2A; Na(+)/Pi cotransporter 2A; NaPi-2a; Solute carrier family 34 member 1; SLC34A1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC34A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 637

• Applicable Applications for SLC34A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC34A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC34A1 sirna

siRNA to inhibit SLC34A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981544
• NCBI GeneID: 25548
• NCBI Accession #: NP_037162.1
• NCBI GenBank Nucleotide #: NM_013030.1
• UniProt Accession #: Q06496
• Molecular Weight: 68,707 Da
• NCBI Official Full Name: sodium-dependent phosphate transport protein 2A
• NCBI Official Synonym Full Names: solute carrier family 34 (type II sodium/phosphate cotransporter), member 1
• NCBI Official Symbol: Slc34a1
• NCBI Official Synonym Symbols: Npt2; NaPi-2; NaPi2A; Slc17a2
• NCBI Protein Information: sodium-dependent phosphate transport protein 2A
• UniProt Protein Name: Sodium-dependent phosphate transport protein 2A
• Protein Family: Sodium-dependent phosphate transport protein
• UniProt Gene Name: Slc34a1
• UniProt Synonym Gene Names: Slc17a2; Sodium-phosphate transport protein 2A; Na(+)/Pi cotransporter 2A; NaPi-2a
• UniProt Entry Name: NPT2A_RAT

NCBI Description

membrane carrier protein that may be involved with active transport of phosphate into cells via sodium cotransport [RGD, Feb 2006]

Uniprot Description

SLC34A1: May be involved in actively transporting phosphate into cells via Na(+) cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx. Defects in SLC34A1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 1 (NPHLOP1). Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization). Defects in SLC34A1 are the cause of Fanconi renotubular syndrome type 2 (FRTS2). It is a disease resulting from a generalized dysfunction of the proximal kidney tubule leading to decreased solute and water reabsorption. Patients have polydipsia and polyuria with phosphaturia, glycosuria and aminoaciduria. They may develop hypophosphatemic rickets or osteomalacia, acidosis and a tendency toward dehydration. Some eventually develop renal insufficiency. Belongs to the SLC34A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family

Cellular Component: cell surface; perinuclear region of cytoplasm; brush border membrane; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; endosome; intrinsic to plasma membrane; lipid raft

Molecular Function: protein homodimerization activity; symporter activity; protein complex binding; sodium-dependent phosphate transmembrane transporter activity; PDZ domain binding

Biological Process: response to drug; indole metabolic process; response to peptide hormone stimulus; ossification; glycoprotein metabolic process; sodium ion transport; bone remodeling; response to estradiol stimulus; response to vitamin A; response to mercury ion; response to magnesium ion; response to cadmium ion; phosphate transport; response to lead ion; phosphate ion homeostasis; positive regulation of membrane potential; kidney development; response to nutrient; protein homooligomerization