SIRPA sirna

SIRPA siRNA (Rat)

Cat. Num. AAA8234178

• Gene Names: Sirpa; Bit; Ptpns1; SHPS-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIRPA; SIRPA siRNA (Rat); BIT; MFR; PTPNS1; SHPS1; SIRP; Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; Signal-regulatory protein alpha-1; Sirp-alpha-1; CD antigen CD172a; SIRPA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SIRPA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 509

• Applicable Applications for SIRPA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SIRPA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIRPA sirna

siRNA to inhibit SIRPA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543529
• NCBI GeneID: 25528
• NCBI Accession #: NP_037148.2
• NCBI GenBank Nucleotide #: NM_013016.2
• UniProt Accession #: P97710; O08951; O70426; Q9QWI5
• Molecular Weight: 55,691 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: Sirpa
• NCBI Official Synonym Symbols: Bit; Ptpns1; SHPS-1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Sirpa
• UniProt Synonym Gene Names: Bit; Mfr; Ptpns1; Shps1; Sirp; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1
• UniProt Entry Name: SHPS1_RAT

NCBI Description

plays a role in macrophage fusion [RGD, Feb 2006]

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion; Cell surface

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein phosphorylated amino acid binding; protein binding; SH3 domain binding

Biological Process: cell migration; positive regulation of phagocytosis; cell-matrix adhesion; actin filament organization; cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; phagocytosis, recognition; phagocytosis, engulfment