SDHD sirna

SDHD siRNA (Rat)

Cat. Num. AAA8215431

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SDHD; SDHD siRNA (Rat); Succinate dehydrogenase [ubiquinone] cytochrome b small subunit mitochondrial; CybS; CII-4; QPs3; Succinate dehydrogenase complex subunit D; Succinate-ubiquinone oxidoreductase cytochrome b small subunit; Succinate-ubiquinone reductase membrane anchor subunit; SDHD sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SDHD siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 159

• Applicable Applications for SDHD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SDHD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SDHD sirna

siRNA to inhibit SDHD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38454310
• NCBI GeneID: 363061
• NCBI Accession #: NP_942083.1
• NCBI GenBank Nucleotide #: NM_198788.2
• UniProt Accession #: Q6PCT8
• Molecular Weight: 16,976 Da
• NCBI Official Full Name: succinate dehydrogenase
• NCBI Official Synonym Full Names: succinate dehydrogenase complex, subunit D, integral membrane protein
• NCBI Official Symbol: Sdhd
• NCBI Protein Information: succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
• Protein Family: Succinate dehydrogenase
• UniProt Gene Name: Sdhd
• UniProt Synonym Gene Names: CybS
• UniProt Entry Name: DHSD_RAT

Uniprot Description

SDHD: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHD are a cause of paragangliomas type 1 (PGL1). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. PGL1 is a rare autosomal dominant disorder which is characterized by the development of mostly benign, highly vascular, slowly growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors. Defects in SDHD are a cause of susceptibility to pheochromocytoma (PCC). A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHD may be a cause of susceptibility to intestinal carcinoid tumor (ICT). A yellow, well- differentiated, circumscribed tumor that arises from enterochromaffin cells in the small intestine or, less frequently, in other parts of the gastrointestinal tract. Defects in SDHD are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHD are a cause of Cowden-like syndrome (CWDLS). Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. Belongs to the CybS family.

Protein type: Membrane protein, integral; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Membrane protein, multi-pass; Energy Metabolism - oxidative phosphorylation

Cellular Component: mitochondrial envelope; mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: ubiquinone binding; metal ion binding; heme binding; succinate dehydrogenase activity

Biological Process: tricarboxylic acid cycle; regulation of catecholamine secretion