RPS14 sirna

RPS14 siRNA (Rat)

Cat. Num. AAA8237437

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPS14; RPS14 siRNA (Rat); 40S ribosomal protein S14; RPS14 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RPS14 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 151

• Applicable Applications for RPS14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RPS14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPS14 sirna

siRNA to inhibit RPS14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12083607
• NCBI GeneID: 29284
• NCBI Accession #: NP_073163.1
• NCBI GenBank Nucleotide #: NM_022672.1
• UniProt Accession #: P13471
• Molecular Weight: 16,259 Da
• NCBI Official Full Name: 40S ribosomal protein S14
• NCBI Official Synonym Full Names: ribosomal protein S14
• NCBI Official Symbol: Rps14
• NCBI Protein Information: 40S ribosomal protein S14
• UniProt Protein Name: 40S ribosomal protein S14
• Protein Family: Ribosomal protein
• UniProt Gene Name: Rps14
• UniProt Entry Name: RS14_RAT

NCBI Description

structural component of the 40S subunit of the ribosome, the organelle responsible for protein synthesis [RGD, Feb 2006]

Uniprot Description

RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Ribosomal; RNA processing; Translation; RNA-binding

Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; ribosome

Molecular Function: structural constituent of ribosome; RNA binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: regulation of translation; translation; ribosomal small subunit assembly and maintenance; erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; maturation of SSU-rRNA