RHOA sirna

RHOA siRNA (Rat)

Cat. Num. AAA8202251

• Gene Names: Rhoa; Arha; Arha2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RHOA; RHOA siRNA (Rat); ARHA; ARHA2; Transforming protein RhoA; RHOA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RHOA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 193

• Applicable Applications for RHOA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RHOA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RHOA sirna

siRNA to inhibit RHOA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16923986
• NCBI GeneID: 117273
• NCBI Accession #: NP_476473.1
• NCBI GenBank Nucleotide #: NM_057132.3
• UniProt Accession #: P61589
• Molecular Weight: 21,782 Da
• NCBI Official Full Name: transforming protein RhoA
• NCBI Official Synonym Full Names: ras homolog family member A
• NCBI Official Symbol: Rhoa
• NCBI Official Synonym Symbols: Arha; Arha2
• NCBI Protein Information: transforming protein RhoA
• UniProt Protein Name: Transforming protein RhoA
• Protein Family: GTP-binding protein
• UniProt Gene Name: Rhoa
• UniProt Synonym Gene Names: Arha; Arha2
• UniProt Entry Name: RHOA_RAT

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein, monomeric; G protein; Oncoprotein

Cellular Component: apical junction complex; focal adhesion; mitochondrion; cell cortex; cytosol; cytoskeleton; membrane; lamellipodium; axon; cytoplasm; plasma membrane; midbody; nucleus; cleavage furrow

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; myosin binding; GTP binding; Rho GDP-dissociation inhibitor binding

Biological Process: radial glial cell differentiation in the forebrain; positive regulation of cell adhesion; positive regulation of translation; metabolic process; cell-matrix adhesion; response to glucocorticoid stimulus; regulation of dendrite development; cell morphogenesis; positive regulation of caspase activity; negative chemotaxis; regulation of cell migration; regulation of osteoblast proliferation; substantia nigra development; small GTPase mediated signal transduction; positive regulation of neuron apoptosis; positive regulation of stress fiber formation; stress-activated protein kinase signaling pathway; response to glucose stimulus; negative regulation of neuron apoptosis; positive regulation of smooth muscle contraction; cell differentiation; cell adhesion; response to drug; positive regulation of cytokinesis; negative regulation of steroid hormone receptor signaling pathway; skeletal muscle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; apical junction assembly; regulation of actin polymerization and/or depolymerization; cytoskeleton organization and biogenesis; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of calcium ion transport; response to amino acid stimulus; positive regulation of cell growth; regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; cerebral cortex cell migration; response to ethanol; positive regulation of actin filament polymerization; response to mechanical stimulus; androgen receptor signaling pathway; response to hypoxia; stress fiber formation; positive regulation of vasoconstriction; positive regulation of neuron differentiation; actin cytoskeleton organization and biogenesis; neurite morphogenesis; positive regulation of cell migration