RALBP1 sirna

RALBP1 siRNA (Rat)

Cat. Num. AAA8221901

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RALBP1; RALBP1 siRNA (Rat); RalA-binding protein 1; RalBP1; Cytocentrin; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1; RALBP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RALBP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 693

• Applicable Applications for RALBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RALBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RALBP1 sirna

siRNA to inhibit RALBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14091752
• NCBI GeneID: 84014
• NCBI Accession #: NP_114456.1
• NCBI GenBank Nucleotide #: NM_032067.1
• UniProt Accession #: Q62796; O55195
• Molecular Weight: 75,252 Da
• NCBI Official Full Name: ralA-binding protein 1
• NCBI Official Synonym Full Names: ralA binding protein 1
• NCBI Official Symbol: Ralbp1
• NCBI Protein Information: ralA-binding protein 1
• UniProt Protein Name: RalA-binding protein 1
• Protein Family: RalA-binding protein
• UniProt Gene Name: Ralbp1
• UniProt Synonym Gene Names: RalBP1; DNP-SG ATPase
• UniProt Entry Name: RBP1_RAT

NCBI Description

binds the GTP-bound form of Ras-related GTPase RalA; also interacts with Rho family member CDC42 via a specific Rho-GTPase activating domain [RGD, Feb 2006]

Uniprot Description

RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Protein type: GAPs, Rac/Rho; GAPs

Cellular Component: spindle pole; neuron projection; membrane; integral to membrane; intracellular; cytosol

Molecular Function: protein binding; ATPase activity, coupled to movement of substances; ATPase activity; Ral GTPase binding; Rac GTPase binding; GTPase activator activity

Biological Process: mitosis; regulation of GTPase activity; mitotic spindle organization and biogenesis; transport; cell division; negative regulation of smooth muscle cell proliferation; metabolic process; small GTPase mediated signal transduction; endocytosis; signal transduction; positive regulation of GTPase activity