RAB3GAP2 sirna

RAB3GAP2 siRNA (Rat)

Cat. Num. AAA8208940

• Gene Names: Rab3gap2; RGD1311518
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB3GAP2; RAB3GAP2 siRNA (Rat); Rab3 GTPase-activating protein non-catalytic subunit; Rab3 GTPase-activating protein 150 kDa subunit; Rab3-GAP p150; Rab3-GAP150; Rab3-GAP regulatory subunit; RAB3GAP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RAB3GAP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1387

• Applicable Applications for RAB3GAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RAB3GAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB3GAP2 sirna

siRNA to inhibit RAB3GAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 281604211
• NCBI GeneID: 289350
• NCBI Accession #: NP_001035244.2
• NCBI GenBank Nucleotide #: NM_001040154.2
• UniProt Accession #: Q5U1Z0
• Molecular Weight: 154,431 Da
• NCBI Official Full Name: rab3 GTPase-activating protein non-catalytic subunit
• NCBI Official Synonym Full Names: RAB3 GTPase activating protein subunit 2
• NCBI Official Symbol: Rab3gap2
• NCBI Official Synonym Symbols: RGD1311518
• NCBI Protein Information: rab3 GTPase-activating protein non-catalytic subunit
• UniProt Protein Name: Rab3 GTPase-activating protein non-catalytic subunit
• Protein Family: Rab3 GTPase-activating protein
• UniProt Gene Name: Rab3gap2
• UniProt Synonym Gene Names: Rab3-GAP150
• UniProt Entry Name: RBGPR_RAT

Uniprot Description

RAB3GAP2: Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3- GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non- synaptic vesicular release of neurotransmitters. Defects in RAB3GAP2 are the cause of Martsolf syndrome (MARTS). Martsolf syndrome is characterized by congenital cataracts, mental retardation, and hypogonadism. Inheritance is autosomal recessive. Defects in RAB3GAP2 are the cause of Warburg micro syndrome type 2 (WARBM2). WARBM2 is a rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism. Belongs to the Rab3-GAP regulatory subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rab; GAPs

Cellular Component: protein complex; cytoplasm; plasma membrane

Molecular Function: protein heterodimerization activity; Rab guanyl-nucleotide exchange factor activity; enzyme regulator activity; Rab GTPase binding; GTPase activator activity

Biological Process: regulation of GTPase activity; positive regulation of GTPase activity