PTK2B sirna

PTK2B siRNA (Rat)

Cat. Num. AAA8211234

• Gene Names: Ptk2b; CAKB; Pyk2; CAKbeta
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTK2B; PTK2B siRNA (Rat); FAK2; PYK2; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; PTK2B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PTK2B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 967

• Applicable Applications for PTK2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PTK2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTK2B sirna

siRNA to inhibit PTK2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148747528
• NCBI GeneID: 50646
• NCBI Accession #: NP_059014.2
• NCBI GenBank Nucleotide #: NM_017318.2
• UniProt Accession #: P70600; O88489; Q3T1H4; Q63201
• Molecular Weight: 111,093 Da
• NCBI Official Full Name: protein-tyrosine kinase 2-beta
• NCBI Official Synonym Full Names: protein tyrosine kinase 2 beta
• NCBI Official Symbol: Ptk2b
• NCBI Official Synonym Symbols: CAKB; Pyk2; CAKbeta
• NCBI Protein Information: protein-tyrosine kinase 2-beta
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: Ptk2b
• UniProt Synonym Gene Names: Fak2; Pyk2; CADTK; CAK-beta; CAKB; FADK 2
• UniProt Entry Name: FAK2_RAT

NCBI Description

may phosphorylate focal adhesion kinase FAK; may play a role in actin cytoskeleton reorganization; may mediate thrombin induced astrocyte proliferation [RGD, Feb 2006]

Uniprot Description

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Nuclear receptor co-regulator; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Fak family

Cellular Component: focal adhesion; postsynaptic density; dendrite; cell cortex; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; cytoskeleton; cell soma; axon; perinuclear region of cytoplasm; lamellipodium; cytoplasm; nucleus

Molecular Function: protein binding; calmodulin-dependent protein kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; 3-phosphoinositide-dependent protein kinase binding; protein complex binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; protein kinase activity; receptor binding

Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; regulation of inositol trisphosphate biosynthetic process; negative regulation of bone mineralization; regulation of cell shape; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; negative regulation of ossification; response to glucose stimulus; negative regulation of neuron apoptosis; cell adhesion; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; actin filament organization; response to osmotic stress; response to organic nitrogen; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to reactive oxygen species; response to ethanol; ionotropic glutamate receptor signaling pathway; response to mechanical stimulus; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; response to cAMP; peptidyl-tyrosine phosphorylation; positive regulation of translation; blood vessel endothelial cell migration; negative regulation of potassium ion transport; response to lithium ion; response to hormone stimulus; regulation of calcium-mediated signaling; positive regulation of JNK cascade; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; angiogenesis; cell differentiation; positive regulation of cell-matrix adhesion; regulation of cell adhesion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; stress fiber formation; response to hypoxia; cellular defense response; innate immune response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration