PPP2R2B sirna

PPP2R2B siRNA (Rat)

Cat. Num. AAA8227105

• Gene Names: Ppp2r2b; Pppr2b2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP2R2B; PPP2R2B siRNA (Rat); Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; PP2A subunit B isoform B55-beta; PP2A subunit B isoform BRB; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A subunit B isoform beta; PPP2R2B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PPP2R2B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for PPP2R2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PPP2R2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP2R2B sirna

siRNA to inhibit PPP2R2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 76880455
• NCBI GeneID: 60660
• NCBI Accession #: NP_071545.2
• NCBI GenBank Nucleotide #: NM_022209.3
• UniProt Accession #: P36877; Q80W84
• Molecular Weight: 51,975 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit B, beta
• NCBI Official Symbol: Ppp2r2b
• NCBI Official Synonym Symbols: Pppr2b2
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: Ppp2r2b
• UniProt Entry Name: 2ABB_RAT

NCBI Description

may regulate the activity of protein phosphatase 2A catalytic subunit; may play a role in spermatid elongation [RGD, Feb 2006]

Uniprot Description

PPP2R2B: the B regulatory subunit of PP2A. May modulate substrate selectivity and catalytic activity, and directs the localization of the catalytic enzyme to particular subcellular compartments. PPP2R2B promotes autophagy and oxidative stress induced cell death that is related to apoptosis. The PP2A holoenzyme consists of a common heterodimeric core enzyme composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits. Three families of regulatory subunits associate with the core dimmer: B (R2/PR55/B55), B¿ (R5/B56), and B¿ (R3/PR72/PR130/PR59). Five isoforms of the human protein are produced by alternative splicing. Isoform 1 (Bbeta1) localizes within the cytoplasm. Isoform 2 (Bbeta2) localizes to both cytosolic and mitochondrial compartments under basal conditions, relocating to the mitochondrial compartment during apoptosis. Its targeting to the outer mitochondrial membrane (OMM) involves an association with import receptors of the TOM complex and is required to promote proapoptotic activity. The N-terminal 26 residues of isoform 2 constitute a cryptic mitochondrial matrix import signal that is necessary and sufficient for targeting the PP2A holoenzyme to the OMM. The last WD repeat of isoform 2 constitutes a mitochondrial stop-transfer domain that prevents PPP2R2B matrix translocation and signal sequence cleavage. Defects in PPP2R2B are the cause of spinocerebellar ataxia type 12 (SCA12).

Protein type: Mitochondrial; Protein phosphatase, regulatory subunit; Apoptosis; Motility/polarity/chemotaxis

Cellular Component: mitochondrial outer membrane; protein phosphatase type 2A complex; cytoskeleton; membrane; mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; protein complex binding; protein phosphatase type 2A regulator activity

Biological Process: neuron differentiation; mitochondrial fission; positive regulation of neuron apoptosis; regulation of protein phosphatase type 2A activity; mitochondrial fragmentation during apoptosis; brain development; signal transduction; protein targeting to mitochondrion; spermatid development