PPP1R10 sirna

PPP1R10 siRNA (Rat)

Cat. Num. AAA8218827

• Gene Names: Ppp1r10; Fb19; Pnuts
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP1R10; PPP1R10 siRNA (Rat); CAT53; PNUTS; Serine/threonine-protein phosphatase 1 regulatory subunit 10; MHC class I region proline-rich protein CAT53; Phosphatase 1 nuclear targeting subunit; Protein PNUTS; PPP1R10 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PPP1R10 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 872

• Applicable Applications for PPP1R10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PPP1R10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP1R10 sirna

siRNA to inhibit PPP1R10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12621126
• NCBI GeneID: 65045
• NCBI Accession #: NP_075240.1
• NCBI GenBank Nucleotide #: NM_022951.2
• UniProt Accession #: O55000; Q6MG09
• Molecular Weight: 92,828 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 1 regulatory subunit 10
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 10
• NCBI Official Symbol: Ppp1r10
• NCBI Official Synonym Symbols: Fb19; Pnuts
• NCBI Protein Information: serine/threonine-protein phosphatase 1 regulatory subunit 10
• UniProt Protein Name: Serine/threonine-protein phosphatase 1 regulatory subunit 10
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: Ppp1r10
• UniProt Synonym Gene Names: Cat53; Pnuts; Protein PNUTS
• UniProt Entry Name: PP1RA_RAT

NCBI Description

functions as nuclear targeting subunit and potent modulator of phosphatase 1 (PP1), a key mediator nuclear processes [RGD, Feb 2006]

Uniprot Description

PNUTS: Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.

Protein type: Protein phosphatase, regulatory subunit

Cellular Component: nucleoplasm; nuclear chromatin; chromatin; nucleus

Molecular Function: DNA binding; RNA binding; metal ion binding; protein phosphatase inhibitor activity; protein phosphatase 1 binding

Biological Process: transcription, DNA-dependent; negative regulation of catalytic activity