PLCG1 sirna

PLCG1 siRNA (Rat)

Cat. Num. AAA8231653

• Gene Names: Plcg1; PPLCA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLCG1; PLCG1 siRNA (Rat); 1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase gamma-1; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PLCG1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1290

• Applicable Applications for PLCG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PLCG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLCG1 sirna

siRNA to inhibit PLCG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981370
• NCBI GeneID: 25738
• NCBI Accession #: NP_037319.1
• NCBI GenBank Nucleotide #: NM_013187.1
• UniProt Accession #: P10686
• Molecular Weight: 148,548 Da
• NCBI Official Full Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• NCBI Official Synonym Full Names: phospholipase C, gamma 1
• NCBI Official Symbol: Plcg1
• NCBI Official Synonym Symbols: PPLCA
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: Plcg1
• UniProt Synonym Gene Names: PLC-gamma-1
• UniProt Entry Name: PLCG1_RAT

NCBI Description

mediates intracellular agonist-induced Ca2+ entry (ACE); serves as a guanine nucleotide exchange factor for the nuclear GTPase PIKE [RGD, Feb 2006]

Uniprot Description

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: Motility/polarity/chemotaxis; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase

Cellular Component: signalosome; ruffle; neuron projection; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: signal transducer activity; glutamate receptor binding; protein binding; neurotrophin TRKA receptor binding; phosphoprotein binding; calcium ion binding; receptor tyrosine kinase binding; phosphoinositide phospholipase C activity; protein kinase binding; insulin receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to gravity; cell migration; phospholipid catabolic process; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; protein secretion; calcium-mediated signaling; response to morphine; signal transduction; response to organic nitrogen; T cell receptor signaling pathway; positive regulation of angiogenesis; response to hydrogen peroxide; inositol trisphosphate biosynthetic process; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol